Journal: Toxics

Article Title: Machine Learning Model for Prediction of Development of Cancer Stem Cell Subpopulation in Tumurs Subjected to Polystyrene Nanoparticles

doi: 10.3390/toxics12050354

Figure Lengend Snippet: Summary of in vitro and in vivo studies using polystyrene particles.

Article Snippet: In vivo : BALB/c nude mice In vitro : human gastric cancer cell lines (AGS, MKN1, MKN45, NCI-N87, and KATOIII) [ ] , purchased from Cospheric (Somis, CA, USA) , polystyrene , 9.5–11.5 μm , In vivo : 1.72 × 10 4 particles/mL In vitro: 8.61 × 10 5 particles/mL , induced resistance to chemo- and monoclonal antibody-therapy.

Techniques: In Vitro, In Vivo, Polymer, Concentration Assay, Mouse Assay

Journal: Scientific Reports

Article Title: Identification and characterization of a vitamin D 3 decomposition product bactericidal against Helicobacter pylori

doi: 10.1038/srep08860

Figure Lengend Snippet: (a) MKN45 cells or T47D cells were incubated for 72 h in the presence of VDP1 at the concentration ranges bactericidal to H. pylori in order to estimate the cell viability by MTT assay. The cell viabilities obtained from experiments performed in triplicate to calculate the mean value ± SD are denoted by the relative A 540 nm in comparison with the value of 1 at the A 540 nm of the viability of the cells incubated without VDP1. (b) Caspase-cleaved keratin 18 (ccK18) was detected in the culture supernatant of T47D cells stimulated for 72 h with either VDP1 or 1α,25-dihydroxyvitamin D 3 (1,25D 3 ). The statistical significance of ccK18 amounts between the cells with or without stimulation was evaluated via the t -test based on data obtained from three independent pair-experiments. N.S. stands for “no statistical significance”. (c) Transient receptor potential vanilloid type 6 (TRPV6) protein was detected in the lysates of T47D cells stimulated with either VDP1 (1 μM) or 1,25D 3 (1 μM). The left-hand panel shows the SDS-PAGE profiles of proteins stained by CBB. The right-hand panel shows the TRPV6 protein profiles detected with anti-TRPV6 antibody. The same amounts of cell lysates were applied to SDS-PAGE in the two panels. Lanes 1, 2, and 3 in the two panels are the protein profiles of un-stimulated cells, 1,25D 3 -stimulated cells, and VDP1-stimulated cells, respectively.

Article Snippet: Human breast cancer T47D cells from DS Pharma Biomedical Co., Ltd. (Osaka, Japan) and human gastric cancer MKN45 cells from GeneticLab Co., Ltd. (Sapporo, Japan) were maintained in an RPMI 1640 (10% FCS-RPMI) medium (Sigma-Aldrich Inc.) supplemented with 10% heat-inactivated fetal calf serum (FCS: Gibco), 10 mM HEPES, 2 mM L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin and 0.2% NaHCO 3 in a humidified chamber at 37°C filled with a 5% CO 2 gas.

Techniques: Incubation, Concentration Assay, MTT Assay, SDS Page, Staining